Propagation of sempur (Dillenia philippinensis Rolfe) using conventional vegetative methods is
considered ineffective due to slow process and low germination level.
Propagation by tissue culture is considered more effective because it does not
depend on the season and requires less plant material. It can benefit sempur
conservation since it is categorized as threatened with extinction in 2020 on
the IUCN red list. This study aimed to evaluate the effect of BAP and NAA on the growth of sempur’s shoots and to observe the morphology of shoot
culture. The media used as a control was MS without growth regulators. The
treatment medium was MS with the addition of BAP and NAA. Shoots were used as
explants. Shoot growth and plantlet morphology were observed eight weeks after planting.
The results indicated that the combination of 1 mg/l BAP and 0.5 mg/l NAA
resulted in the highest total number of leaves, nodes and adventitious shoots.
The combination of BAP and NAA, each 1 mg/l, resulted in the highest fresh
weight and callus formation. The combination of 1 and 2 mg/l BAP and 0.5-1 mg/l
NAA could not form roots. In comparison, the combination of BAP and NAA in
culture media could change the shape and size of the leaves. The survival rate
of growth of plantlets derived from MS medium was 50% at 22 weeks after
acclimatization.
Rantau, D. E., Wulandari, D. R., Ermayanti, T. M., Rudiyanto, R., Hapsari, B. W., Wulansari, A. & Firdaus, H. L. Pertumbuhan Dan Morfologi Kultur Tunas Sempur (Dillenia philippinensis Rolfe) Pada Media MS-BAP-NAA. Jurnal Penelitian Hutan Tanaman, 18 (1):65-78. DOI: https://doi.org/10.20886/jpht.2021.18.1.65-78
