Moringa
(Moringa oleifera Linn) has a highly considerable nutritional value. The
conventional propagation of Moringa has limitation. Propagation through tissue
culture offers uniform transplants, and free of pests and diseases. Selection
of basic medium is a critical point to find out the best medium compositions
for micropropagation. The research was aimed to investigate growth response of M. oleifera shoots
cultured on several basic media. The experimental design was factorial complete
random design with two factors i.e. types of basic medium (MS, WPM, DKW, NN,
and B5) in combination with accessions of M. oleifera:
Bogor, Pekalongan, Bima and Blora. Each treatment consisted of 12 replicates.
The growth variables observed were shoot height, number of shoots, number of
petioles, number of roots, fresh weight, dry weight of plantlets, and plantlet
performance. The growth observation was carried out every week. The results
showed that type of basic medium significantly affected shoot height, number of
petioles, fresh weight, dry weight, and number of shoots, but, it did not
significantly affect the number of roots. The accessions significantly affected
shoot height, number of petioles, number of roots, fresh weight, and dry
weight, but, did not significantly affect number of shoots. The ANOVA showed an
interaction between basic medium in combination with accessions on all growth
variables observed. The highest shoot height (13,97 cm), number of petioles
(12,33), and number of roots (8.33) were achieved on DKW basic medium on Bima
and Pekalongan accessions, while the highest number of shoots (6.33) was found
in NN medium.
Rudiyanto, Purwito, A.,
Efendi, D., & Ermayanti, T. M. (2021). Growth response of four accessions
of Moringa oleifera Linn shoots cultured on various basic media. In IOP Conference Series: Earth and Environmental Science (Vol. 741, No. 1, p. 012054). IOP Publishing.
Link DOI: https://doi.org/10.1088/1755-1315/741/1/012054
