Induksi Poliploid Tacca leontopetaloides Linn Secara in vitro dengan Orizalin

Taka plant (Tacca leontopetaloides Linn.) which produces tubers useful for a source of carbohydrates. In Indonesia, this plant grows only at coastal areas. Tissue culture has been developed for conservation and mass propagation in many plant species. Increasing genetic diversity and plant productivity can be achieved by polyploidization. The aim of this research was to induce Taka polyploid plants in vitro culture using oryzalin. Experimental design was Completely Randomized Design with factors tested were 0, 7.5, 15, 30, 60 and 75 μM orizalyn. Every treatment has 20 replicates. The variables observed were: shoot height, number of leaves and number of roots which were observed weekly at 1-4 weeks after culture. Flowsitometer analysis was done to confirm of Taka ploidy. The results showed that shoot height and number of leaves on 0, 7.5, 15, 30, 60 and 75 μM of orizalyn were not significantly different. The highest number of roots was produced by control and 30 μM of oryzalin. Additional of 15 and 75 μM oryzalin produced highest tetraploid shoots. Mixoploid shoots were found with 75 μM oryzalin. Triploid shoots were only produced by 75 μM oryzalin, while hexaploid and octaploid shoots were produced by 7.5 and 15 μM oryzalin, respectively.

Martin, A. F., Rudiyanto, B. W. H., & Ermayanti, T. M. (2018). INDUKSI POLIPLOID TACCA LEONTOPETALOIDES LINN SECARA IN VITRO DENGAN ORIZALIN. Prosiding SemNas “Biodiversitas Untuk Kehidupan”; Jakarta.

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