Micropropagation of diploid and tetraploid Plectranthus amboinicus (Lour.) Spreng

 

Plectranthus amboinicus (Lour.) Spreng is commonly consumed by people in North Sumatra, Indonesia, as a vegetable, which is commonly called Torbangun. Plant tissue culture is an alternative method for the mass-production of transplants. Genetic improvement by somatic cell manipulation such as induction of polyploid plants is needed to improve biomass production as well as their secondary metabolite products. This research aimed to conduct in vitro micropropagation of diploid and tetraploid P. amboinicus on Murashige and Skoog (MS) medium containing BAP or Kinetin. Shoot tips were used as explants and cultured on MS medium containing BAP or Kinetin at 0, 0.5, 1 and 2 mg/l given as single concentration. The experiment used a completely randomized design with four replications. Each replication consisted of three explants. The results showed that height of shoots was affected by type of cytokinins. Kinetin at 0.5 mg/l gave the highest shoots of tetraploid plants significantly different with addition of BAP both to diploid and tetraploid plants. The number of multiple shoots and leaves varied in both diploid and tetraploid plants. Root formation was best on the medium without addition of cytokinins. Both diploid and tetraploid plantlets had a high survival rate after acclimatization in the greenhouse


Sari, L., Rudiyanto, Hafiizh, E. A., & Ermayanti, T. M. (2021, May). Micropropagation of diploid and tetraploid Plectranthus amboinicus (Lour.) Spreng. In IOP Conference Series: Earth and Environmental Science (Vol. 762, No. 1, p. 012040). IOP Publishing.


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Induksi Poliploid Tacca leontopetaloides Linn Secara in vitro dengan Orizalin

Taka plant (Tacca leontopetaloides Linn.) which produces tubers useful for a source of carbohydrates. In Indonesia, this plant grows only at coastal areas. Tissue culture has been developed for conservation and mass propagation in many plant species. Increasing genetic diversity and plant productivity can be achieved by polyploidization. The aim of this research was to induce Taka polyploid plants in vitro culture using oryzalin. Experimental design was Completely Randomized Design with factors tested were 0, 7.5, 15, 30, 60 and 75 μM orizalyn. Every treatment has 20 replicates. The variables observed were: shoot height, number of leaves and number of roots which were observed weekly at 1-4 weeks after culture. Flowsitometer analysis was done to confirm of Taka ploidy. The results showed that shoot height and number of leaves on 0, 7.5, 15, 30, 60 and 75 μM of orizalyn were not significantly different. The highest number of roots was produced by control and 30 μM of oryzalin. Additional of 15 and 75 μM oryzalin produced highest tetraploid shoots. Mixoploid shoots were found with 75 μM oryzalin. Triploid shoots were only produced by 75 μM oryzalin, while hexaploid and octaploid shoots were produced by 7.5 and 15 μM oryzalin, respectively.


Martin, A. F., Rudiyanto, B. W. H., & Ermayanti, T. M. (2018). INDUKSI POLIPLOID TACCA LEONTOPETALOIDES LINN SECARA IN VITRO DENGAN ORIZALIN. Prosiding SemNas “Biodiversitas Untuk Kehidupan”; Jakarta.
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Copyright (c) 2024 |Dr. Rudiyanto, SP., M.Si.|Associate Researcher at Research Center for Applied Botany BRIN, Indonesia