Moringa oleifera Linn. is a plant with significant potential as a
functional food. For mutation, the gamma rays irradiation has been proven
effective in producing prominent characteristics such as tolerance to aluminum
(Al) stress. Therefore, the present study aimed to assess the genetic
variability of M. oleifera plant by
exposing to gamma rays irradiation at various doses. Selection of Al tolerant
genotypes was carried out in in vitro under Al stress conditions by adding AlCl3
to the media at the rate of 0, 50, 100, 250, and 500 mg/L. The selection was
made for prominent accessions of M.
oleifera capable of producing high yields under abiotic stress conditions.
In addition to agronomic parameters, the organic acids content were observed to
select the tolerant accessions with metabolic profile of Al stress tolerant
accessions. Gamma irradiation applied to M.
oleifera shoot culture produced new traits, as shown by the molecular
dendrogram of ISSR markers, where the 10 and 20 Gy treatments had varied
genetic diversity compared to the wild type. Additionally, gamma irradiation at
10 and 20 Gy increased the tolerance of M.
oleifera culture to 100 and 250 mg/L of AlCl3.Rudiyanto, Purwito A, Efendi D,
Martin AF (2025). Moringa oleifera
aluminum tolerance produced by gamma irradiation through in vitro culture.
SABRAO J. Breed. Genet. 57(2): 708-718.
Link DOI: http://doi.org/10.54910/sabrao2025.57.2.27.
