Moringa (
Moringa
oleifera Linn.) is a popular plant
as a functional food crop. In micropropagation, modifying the medium culture is
critical to determine the best nutrient composition for plantlet production.
The antecedent research showed that the basic DKW (Driver and Kuniyaki Walnut)
medium was the best compared to MS (Murashige and Skoog), WPM (Woody Plant
Medium), NN (Nitsch and Nitsch), and B5 (Gamborg) media. This experiment aimed
to enhance the growth of shoot culture and its root formation on a basic DKW
medium supplemented with cytokinins and auxins. Two experiments were conducted.
The first experiment was designed to obtain the best medium for shoot growth by
adding cytokinin BAP, Kinetin, 2-IP, and Zeatin. The use of IAA
(indole-3-acetic acid), NAA (1-naphthalene acetic acid), and IBA
(3-indolebutyric acid) to enhance root formation was set for the second
experiment. A completely randomized design with two elements was utilized for
the experiment. The variables tried were: Plant Growth Regulators (PGR): BAP,
Kinetin, 2-IP, and Zeatin with 0.0, 0.5, 1.0, and 2.0 mg/l for shoot
development and IAA, NAA, and IBA at a similar concentration for root
arrangement. Every treatment comprised of 12 recreates. The factors noticed
were shoot stature, shoot numbers, petiole numbers, root numbers, and root
length noticed one time per week. Following a month and a half after culture,
plantlet performance were recorded. The best shoot stature, shoot numbers, and
petiole numbers were created on a medium enhanced with 1 mg/l Kinetin and significantly
unique with others. The most significant roots and root length were created at
1.0 and 2.0 mg/l IAA.
Rudiyanto, R., Purwito, A., Efendi, D., &
Ermayanti, T. M. (2023, January). In vitro shoot growth and root formation
enhancement of Moringa oleifera Linn. on DKW medium containing cytokinins and
auxins. In AIP Conference Proceedings (Vol. 2606, No. 1). AIP Publishing.
Link DOI: https://doi.org/10.1063/5.0118345
