Moringa oleifera Lam., a
promising functional food, can be propagated rapidly through somatic
embryogenesis technique. This method has advantages, such as rapid seed
generation, absence of a dormant phase, and bipolar cotyledons. The induction
of M. oleifera callus involves plant growth regulators, the type of
explants, and the media used. In this study, we investigated the effects of
0.0, 0.5, 1.0 and 2.0 mg/L of BAP and picloram on inducing embryonic callus in M.
oleifera plants. Additionally, we examined the impact of amino acids such
as proline, glutamine, and cysteine on the proliferation stage of the embryonic
callus. The results showed that 1 mg/L BAP and picloram significantly enhanced
callus induction, as indicated by increased callus diameter, weight, and
volume. The growth of M. oleifera callus was also influenced by the
concentration of amino acids. The optimal proliferation callus was achieved
using 200 mg/L of proline in Driver and Kuniyuki Walnut (DKW) medium with the
Temporary Immersion System (TIS). Furthermore, embryogenic callus was generated
using 200 mg/L proline, 50 mg/L glutamine, and 50 and 100 mg/L cysteine. The
addition of 200 mg/L proline to DKW yielded the best results in TIS.
Rudiyanto, Purwito A, Efendi D,
Martin
AF. 2024. Induction and proliferation of Moringa
oleifera somatic embryo callus using solid liquid and temporary immersion
system.
IJAB 32(3):294-300.
Link
DOI: https://doi.org/10.17957/IJAB/15.2204
