Pertumbuhan Dan Morfologi Kultur Tunas Sempur (Dillenia philippinensis Rolfe) Pada Media MS-BAP-NAA

Propagation of sempur (Dillenia philippinensis Rolfe) using conventional vegetative methods is considered ineffective due to slow process and low germination level. Propagation by tissue culture is considered more effective because it does not depend on the season and requires less plant material. It can benefit sempur conservation since it is categorized as threatened with extinction in 2020 on the IUCN red list. This study aimed to evaluate the effect of BAP and NAA on the growth of sempur’s shoots and to observe the morphology of shoot culture. The media used as a control was MS without growth regulators. The treatment medium was MS with the addition of BAP and NAA. Shoots were used as explants. Shoot growth and plantlet morphology were observed eight weeks after planting. The results indicated that the combination of 1 mg/l BAP and 0.5 mg/l NAA resulted in the highest total number of leaves, nodes and adventitious shoots. The combination of BAP and NAA, each 1 mg/l, resulted in the highest fresh weight and callus formation. The combination of 1 and 2 mg/l BAP and 0.5-1 mg/l NAA could not form roots. In comparison, the combination of BAP and NAA in culture media could change the shape and size of the leaves. The survival rate of growth of plantlets derived from MS medium was 50% at 22 weeks after acclimatization.

Rantau, D. E., Wulandari, D. R., Ermayanti, T. M., Rudiyanto, R., Hapsari, B. W., Wulansari, A. & Firdaus, H. L. Pertumbuhan Dan Morfologi Kultur Tunas Sempur (Dillenia philippinensis Rolfe) Pada Media MS-BAP-NAA. Jurnal Penelitian Hutan Tanaman, 18 (1):65-78. DOI: https://doi.org/10.20886/jpht.2021.18.1.65-78

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Detection of Somaclonal Variation in Jatropha curcas Linn. Plantlets Regenerated from Somatic Embryo using ISSR Markers

Physic nut (Jatropha curcas Linn.) has the potential as a source of sustainable biofuels. Somatic embryo proliferation of J. curcas may cause somaclonal variations. This research aimed to investigate somaclonal variations of J. curcas somatic embryo derived-plantlet using ISSR markers. Somatic embryos of J. curcas at the globular phase were cultured on liquid MS medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg L-1 of 2,4-D. Parameter observed were embryos weight, embryos volume, colour, and size of embryos. After proliferation, the embryos were cultured on a germination medium until the cotyledonary phase. The plantlet’s leaves were used for DNA samples for ISSR analysis. The results showed that proliferation of J. curcas somatic embryos was optimal at MS medium supplemented with 1 mg L-1 2,4-D and produced the highest weight and volume of embryos. The furthest genetic distance occurred between the control and J. curcas plantlet which was regenerated from MS + 1 mg L-1 2,4-D which had 0.60 of similarity coefficient.

Rudiyanto, Efendi, D., Al-Hafiizh, E., & Ermayanti, T. M. (2021). DETEKSI VARIASI SOMAKLONAL PLANLET Jatropha curcas Linn. HASIL REGENERASI EMBRIO SOMATIK DENGAN MARKA MOLEKULAR ISSR. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 8(1), 14-24.

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